dna template (New England Biolabs)
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New England Biolabs
dna template
Dna Template, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+template/T7+RNA+Polymerase/pm42087788-37-32-41
Average 99 stars, based on 2612 article reviews
Dna Template, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+template/T7+RNA+Polymerase/pm42087788-37-32-41
Average 99 stars, based on 2612 article reviews
dna template - by Bioz Stars,
2026-11
99/100 stars
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In Vitro:Article Title: Population dynamics of ribozymes during in vitro selection Article Snippet: .. In vitro transcription reactions contained 40 mM Tris-HCl (pH 8), 2 mM spermidine, 10 mM NaCl, 25 mM MgCl 2 , 10 mM Dithiothreitol (DTT), 30 U/mL RNase inhibitor murine (NEB), 2.5 U/mL thermostable inorganic pyrophosphatase (TIPPase) (NEB), 4 mM of each NTP, 30 pmol/mL Article Title: Omnivore RNA pyrophosphatase: a versatile enzyme that efficiently removes diverse protecting groups from the 5' end of RNA. Article Snippet: .. In vitro transcription was performed for 12 h at 37◦C in a reaction mixture (50 μl) containing Tris–HCl, pH 7.9 (40 mM), MgCl2 (6 mM), spermidine (2 mM), dithiothreitol (1 mM), the Article Title: Loop engineering of AtCas9 for effective and broad genome editing Article Snippet: .. Each 50 μL in vitro transcription reaction contained 0.5–1 μg of Article Title: Primate-specific sperm lnc-CLCN7 reveals embryo quality in IVF. Article Snippet: .. A digoxigenin (Dig)-labeled single-stranded RNA probe was transcribed in vitro from a T7 promoter-containing Article Title: ApaH decaps Np 4 N-capped RNAs in two alternative orientations. Article Snippet: Enigmatic dinucleoside tetraphosphates, known as ‘alarmones’ (Np4Ns), have recently been shown to function in bacteria as precursors to Np4 caps on transcripts, likely influencing RNA longevity and cellular adaptation to stress.. In proteobacteria, ApaH is the predominant enzyme that hydrolyzes Np4Ns and decaps Np4-capped RNAs to initiate their 5′-end-dependent degradation.. Here we conducted a biochemical and structural study to uncover the catalytic mechanism of Escherichia coli ApaH, a prototypic symmetric Np4N hydrolase, on various Np4Ns and Np4-capped RNAs. Concentration Assay:Article Title: Omnivore RNA pyrophosphatase: a versatile enzyme that efficiently removes diverse protecting groups from the 5' end of RNA. Article Snippet: .. In vitro transcription was performed for 12 h at 37◦C in a reaction mixture (50 μl) containing Tris–HCl, pH 7.9 (40 mM), MgCl2 (6 mM), spermidine (2 mM), dithiothreitol (1 mM), the Article Title: ApaH decaps Np 4 N-capped RNAs in two alternative orientations. Article Snippet: Enigmatic dinucleoside tetraphosphates, known as ‘alarmones’ (Np4Ns), have recently been shown to function in bacteria as precursors to Np4 caps on transcripts, likely influencing RNA longevity and cellular adaptation to stress.. In proteobacteria, ApaH is the predominant enzyme that hydrolyzes Np4Ns and decaps Np4-capped RNAs to initiate their 5′-end-dependent degradation.. Here we conducted a biochemical and structural study to uncover the catalytic mechanism of Escherichia coli ApaH, a prototypic symmetric Np4N hydrolase, on various Np4Ns and Np4-capped RNAs. Generated:Article Title: The structure and catalytic mechanism of new cellular and viral HDV ribozymes Article Snippet: .. The longer (ribozyme) strands of both ribozymes divided at the J1/2 connection were generated by T7 RNA polymerase transcription from a Polymerase Chain Reaction:Article Title: The structure and catalytic mechanism of new cellular and viral HDV ribozymes Article Snippet: .. The longer (ribozyme) strands of both ribozymes divided at the J1/2 connection were generated by T7 RNA polymerase transcription from a Labeling:Article Title: Primate-specific sperm lnc-CLCN7 reveals embryo quality in IVF. Article Snippet: .. A digoxigenin (Dig)-labeled single-stranded RNA probe was transcribed in vitro from a T7 promoter-containing Fluorescence:Article Title: Fast-Track Detection of Chilli Leaf Curl Virus using CRISPR/Cas12a without Amplification Article Snippet: Chilli Leaf Curl Virus (ChLCV), a monopartite begomovirus, is the primary viral cause of Chilli leaf curl disease.. This disease severely impacts chilli farming in India, often destroying entire yields.. Employing rapid, specific, and sensitive techniques for early detection is vital to developing and evaluating effective disease management strategies. Synthesized:Article Title: ApaH decaps Np 4 N-capped RNAs in two alternative orientations. Article Snippet: Enigmatic dinucleoside tetraphosphates, known as ‘alarmones’ (Np4Ns), have recently been shown to function in bacteria as precursors to Np4 caps on transcripts, likely influencing RNA longevity and cellular adaptation to stress.. In proteobacteria, ApaH is the predominant enzyme that hydrolyzes Np4Ns and decaps Np4-capped RNAs to initiate their 5′-end-dependent degradation.. Here we conducted a biochemical and structural study to uncover the catalytic mechanism of Escherichia coli ApaH, a prototypic symmetric Np4N hydrolase, on various Np4Ns and Np4-capped RNAs. |